Tam sim no, lub ntiaj teb tiagBromelain hmoovcov tuam txhab lag luam feem ntau nyob hauv Thaib teb thiab Indonesia. Chongqing Joywin Natural Products Co., Ltd. yog ib lub tuam txhab tseem ceeb nrog crude enzyme chaw nyob hauv Prachuapkhirikhan Thaib teb, ib yam nkaus. Nyob rau hauv xyoo tas los no, vim tias kev lag luam nce ntxiv ntawm bromelain, tau muaj qhov tsis txaus ntawm cov khoom siv. Thiab cov nyiaj ntau ntawm Indian "Bromelain" nkag mus rau lub ntiaj teb ua lag luam ntawm tus nqi qis heev. Tus nqi yog ntawm US $ 20 ~ $ 60 / kg rau 2400GDU "Bromelain".
Tom qab kev tshawb nrhiav, nws tau pom tias tsis muaj cov chaw tsim khoom tsim bromelain hauv Is Nrias teb. Thaum pib thawj zaug, Is Nrias teb "bromelain" txoj kev sim kev ua si yog feem ntau raws li cov qauv kev lag luam, thiab cov tuam txhab Indian no tau dag rau txoj kev sim los ntawm kev hloov pauv hloov pauv hauv cov mis kom ua tiav cov txiaj ntsig kev ua haujlwm enzyme.
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Hom |
Is Nrias teb "Bromelain" chaw tsim tshuaj paus Assay |
International Common Assay |
Memo |
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Assay los ntawm |
Indian chaw tsim tshuaj paus GDU Assay |
International Common GDU Assay |
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Zoo sib xws |
Substrate |
Gelatin |
Gelatin |
Siv tib lub substrate |
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Lub ntsiab lus ntawm kev ua enzyme |
Unit Txhais (Gelatin Digestion Unit-GDU): Ib chav yuav hydrolyze 1.0mg ntawm amino nitrogen los ntawm gelatin hauv 20 feeb ntawm pH 4.5 ntawm 45 degree . |
Unit Txhais (Gelatin Digestion Unit-GDU): Ib chav yuav hydrolyze 1.0mg ntawm amino nitrogen los ntawm gelatin hauv 20 feeb ntawm pH 4.5 ntawm 45 degree . |
GDU enzyme kev ua haujlwm txhais tib yam |
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Cov kauj ruam xeem |
Siv cov tshuaj NaOH rau kev kuaj titrant |
Siv cov tshuaj NaOH rau kev kuaj titrant |
Txoj kev xeem tseem ceeb yog tib yam |
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Qhov txawv |
Titrant concentration |
0.05mol/L NaOH Titrant |
0.1mol/L NaOH Titrant |
Qhov concentration ntawm NaOH titrant tov ntawm kev tshuaj ntsuam GDU yog ob npaug tshaj qhov kev xeem Indian GDU. |
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End point ntawm titrant |
Xaus point PH 7.8 |
End point PH 9.0 |
Tus nqi pH sib txawv ntawm qhov kawg taw tes txheeb xyuas |
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Formula |
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Cov mis nyuj sib txawv |
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Indian Assay: Tsis muaj lus piav qhia rau cov qauv kev suav Raws li cov txheej txheem kuaj thiab lub ntsiab lus ntawm kev ua enzyme peb xav tias: T{0}} Test titer (ml 0.05 N NaOH) B{{0}} Blank titer (ml 0.05 N NaOH) ml 0 1- Qhov hnyav pib ntawm enzyme (g) |
T{0}} Test titer (ml 0.1 N NaOH) ml B{{0}} Blank titer (ml 0.1 N NaOH) ml N=Ntau tus qauv NaOH (mol/L) M=Pib qhov hnyav ntawm enzyme (g) 14-Molecular hnyav ntawm nitrogen; 50-Dlution ratio ntawm tus qauv enzyme |
Raws li Indian chaw tsim tshuaj paus Assay, thiab ua ke nrog thoob ntiaj teb hom GDU enzyme kev ua haujlwm txhais, tus lej 700 hauv cov qauv yuav tsum yog (14 × 0.05 × 100=70), tab sis lawv kos nws li 700. Qhov no ua rau qhov tshwm sim tsis ncaj ncees uas yog 10 npaug ntxiv. |
Nrog ntau thiab ntau cov neeg siv khoom pom tau tias qhov no yog qhov kev ntsuam xyuas tsis raug thiab pom tias qhov kev ua haujlwm tiag tiag tsuas yog ib feem kaum ntawm cov haujlwm tiag tiag, cov tuam txhab Indian tau pib muab papain nrog lwm cov enzymes li bromelain. Raws li GDU kev txheeb xyuas ntau npaum li cas ntawm ob qho tib si papain thiab bromelain yog tib yam, Indian "bromelain" ntawm kev ua lag luam yog tsim nyog nrog cov enzyme kev ua. Txawm li cas los xij raws li cov papain uas tsim nyob rau hauv Is Nrias teb muaj stink tsw ntawm protein fermentation, lawv feem ntau siv flavors thiab fragrances los npog qhov tsw. Qhov no ua rau Indian "Bromelain" nrog ob qho tib si fermentation tsw thiab tsw tsw. Thaum qhov tseeb ntshiab bromelain muaj qhov tshwj xeeb fragrant tsw ntawm pineapple qia.
Kev txheeb xyuas ntawm Bromelain
1. SDS-PAGE Analysis
Mark: M-protein marker, 1-Bromelain(Joywin)(10μL); 2-Bromelain (India chaw tsim tshuaj paus) (10μL); 3-Bromelain(India)(10μL); 4-Papain (10μL)
Qhov hnyav molecular ntawm Protein Marker bands los ntawm sab saum toj mus rau hauv qab hauv kab lus yog:
180 Kda, 130 Kda, 100 KDa, 70 KDa, 55 KDa, 40 KDa, 35 KDa, 25 KDa, 15 KDa, thiab 10 KD.
Cov txiaj ntsig ntawm SDS-PAGE Analysis qhia tau hais tias cov yam ntxwv ntawm bromelain yog kwv yees li 25Kda, muaj 3 qhov tseem ceeb ntawm bromelain, thaum papain muaj ntau pawg ntawm 13 ~ 30.
2. MALDI-TOF MS Identification
Los ntawm MALDI-TOF MS chromatogram tuaj yeem pom, bromelain tsuas muaj ib tus cwj pwm ncov, ntawm txog 24.5Kda, thaum papain muaj peb lub cim ncov, nyob ib ncig ntawm 13.4Kda, 23.7Kda thiab 26.7Kda. Nws ntxiv qhia tias cov ntsiab lus ntawm Indian "bromelain" yog papain.
JOYWINnrhiav tau nyob rau hauv 2013 yog lub tuam txhab innovation-driven biotechnology. Lub Hoobkas JOYWIN Bromelain nyob rau Thaib teb ua rau siv cov khoom siv hauv zos los muab cov neeg siv khoom nrog ntau yam tshwj xeeb ntawm cov khoom bromelain. Los ntawm 200GDU / g rau 2400GDU / g. Tuav cov kev cob qhia bromelain, cog kev cob qhia protease, thiab warehouses kuj muaj cov chaw txiav-ntug thiab kev tswj xyuas nruj. Raws li ib qho ntawm plaub lub ntiaj teb bromelain manufacturers, peb yog FSSC22000, ISO9001, ISO14001, ISO22000, BRC, thiab Cgmp-certified Hoobkas. Yog koj xav paub ntxiv txogBromelain hmoovlos yog xav yuav nws, koj tuaj yeem email ntawmcontact@joywinworld.com. Peb yuav teb koj sai li sai tau tom qab peb pom cov lus.